No tools required to assemble. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl. Product Compare 0. Molecular Devices (UK) Ltd. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). • Gently wash cells once with 200 μl (for 96 well plate) or 400 μl (for a 24 well plate) warm (37°C) 1X DPBS (containing Mg and CaCl 2). The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. of Dispensers:Molecular Devices deliver high throughput while showing good correlation with manual. 005 OD Normal and Rapid modes, 96- and 384-well plates Fluorescence Read Timing Excitation Filter: 485/20 nm Emission Filter: 528/20 nm 96-well read: 89 sec. No additional manuals are available. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Molecular Devices SpectraMax Plus Microplate Reader Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader Sale Price: $6,495. 300 µL of Detergent Compatible Bradford reagent was added to each well. For a 96-well plate it takes a total of 27 seconds to switch between wells and then you have to add the actual injection time based on the injection rate entered by the user. The plate reader can measure multiple readouts, including UV and. Quartz 96 Well Plates | 96 Well Plate Flat Bottom | 96 Well Plate Reader | 96 Well Glass. 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. No accessories are needed and SoftMax® Pro Software. Read up to 2 wavelengths in endpoint absorbance for 96-well plates. Service parts for these models are not being produced and are no longer in stock. Flexible liquid transfer Using an 8- or 16-channel pipettor, the FlexStation 3 Reader offers added assay flexibility over dispenser-based systems by transferring reagents from 96 or 384 distinct wells in a source plate to a read plate, simultaneously. BMG Labtech - THERMOstar. Features of Streptavidin Coated Plates include: • Approximate binding capacity is ∼5 pmol biotin/well. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 000 – 4. Figure 2. Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Molecular Devices. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. Temperature control: 4°C above ambient to 45°C. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Performing molecular and cellular analyses in multiwell microplate formats is an important methodology for high-throughput screening, or for boosting throughput generally. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Top reading gives greater sensitivity for 96 well plates, unless you have cells adhering to the bottom of the well. Dynamic range > 6 decades . UV cuvettes are ideally suited for measurements at 260 nm, 280 nm and in the visible range. Two high-energy xenon flash lamps: Reading capabilities. Molecular Devices: FlexStation 3: Multimodal: Limited - monochronometer based: N/A:the iD5 reader’s filter system. 5 pM 1 0200-7015POS Glow for 96- and 384-well plates 96–well: 1. Spectramax ® Plus microtiter plate reader (Molecular Devices, Sunnyvale, CA) or similar UV-Vis plate reader; SoftMax ® Pro software (Molecular Devices) (optional) UV compatible quartz plate (for example: # R8024; Molecular Devices) or 96-well Costar ® with UV transparent bottom (for example: #3635 ; Corning Inc. One plate received 200 µL of the BCA working reagent, and the other received 200 µL of the Rapid Gold BCA working reagent. Primary considerations when choosing a microplate reader are throughput and flexibility. FlexIII. For the kinetic and endpoint LAL assays, the ELx808 TM Reader is highly reliable and easy to use. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. Two high-energy xenon flash lamps: Reading capabilities. With theMolecular DevicesWe designed and fabricated different sized microfluidic covers, matched to the desired experimental conditions: Fig. Step 1: Capture antibody binds to ELISA plate wells. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm: Measurement Type: Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra: No. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. LogPhase 600 is controlled with a software App that provides a simple, yet powerful, user experience. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Making it possible to perform kinetic analysis of solid. SpectraMax® Microplate Reader Suite of Products Knowledge Base. A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only). The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). Download a PDF version of the SpectraTest validation package User Guide and the ABS2 SpectraTest validation protocols. The. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. 057, depending on lot number. Robust shaking and consistent temperature control optimize bacteria and yeast cell growth. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. Determine concentration using the Beer-Lambert LawPage 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. The instrument reads any standard cuvette, microcuvette, test tube and 96 well or UV transparent microplate over full 190-1000 nm wavelength range. • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettorElisa Plate Readers and Microplate Readers at Biocompare. View Product. Category 2 and 3 microplate readers use gradient heating as well. Next generation SpectraMax® absorbance microplate readers. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. T = I/I 0 and %T = 100 (T). 5 second read per plate. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Plus 384. 314 low lamp level warning. All trademarks used herein are the property of. Microplates, cuvettes (via adapter) Detectors. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Four wash/rinse bottles are included and configurable for both 96- and 384. The VersaMax reads 96-well microplates. Devices® SPECTRAmax 190 microplate reader at a wavelength of 600 nm. 4ti-0255 96 well assay plate, pyramid flat bottom, clear, non-sterile UV wavelength 4ti-0214 UltraVision Plate 384, black, non -sterile, with UV baseELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. Optimized for fluorescence microplate readers. Cells were transfected as previously described and plated at 30,000 cells per well in a 96-well plate and grown overnight. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. Two days before the experiments, cells are seeded into the clear-bottom 96-well plates (approximately 10,000 cells per well for cancer cell lines and 30,000–40,000 cells per well for fibroblasts and NPC; see Note 5) and cultured in the media suited for users’ cell lines. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. 7°C (gradient heating design). Mode Plate Reader. SpectraDrop High-Throughput Screening (HTS) Kit. An industry-renowned instrument for monitoring GPCRs and ion channels. HeLa. Molecular Devices delivers a robust screening platform to address real-time kinetic cell-based assays with the FLIPR Tetra® System. Reaction formats The MagMAX™-96 DNA Multi-Sample Kit can accommodate a broad range of throughputs. Absorbance was then determined at 450 nm by SpectraMax Paradigm microplate reader (Molecular. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. 97 independent and fixed optical fibers can achieve a mean time between failures (MTBF) of 20,000 hours, which keeps the microwell plate still during the entire reading cycle,. x. 0 . The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well…. 00 . Products Clone Screening Accessories and Consumables Deep-well Plates Deep-well Plates Deep well blocks available in 24, 96 and 384-well formats Product Options. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Related Products: 96 Well Plate Reader. Detection cartridge modularity allows for easy addition of new read modes within minutes without. 4 (Molecular Devices cat. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. 25, 3. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate: 0517 Lit. , Corning, NY) or Greiner. 5 fg per well to 0. 00. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. 0200-6262. Lucid Scientific. UV Plate 96 Well, Flat Bottom, Cs 50. Corning. one 96-well sample plate can be controlled from 15–40°C,. 00. x. Another consideration, says Amouretti, is plate format. Working in. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. Compatible with Molecular Devices SPECTRAmax 190 and 250 plate readers. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…AquaMax® Microplate Washers are configurable with user interchangeable heads for both 96- and 384-well microplate washing applications, including plate or cell washing. Molecular Devices' microplate readers. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e. 1c shows a cover designed to perfuse 8 wells in series on a standard 96-well microplate, as well as a cover for the entire standard 96-well microplate. These are the default kind of cuvette & 96 well plate stocked by the lidstrom lab as of 11/2013. Usage/Application: Lab. Or scan up to 384 wells at any time to determine the best assay conditions. Compare this item. Important things to consider when purchasing a multimode microplate reader. The 8-channel read head allows for fast read times which means a 96-well plate can be read in as little as five seconds. 100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. 96-, 384-, and 1536-well microplates. Fluorescence Microplate Reader Comparison Chart. White 96-well plate Plate formats. This instrument is ideal for measuring dual luciferase assays in both 96- and 384-well plates, and SoftMax Pro® software simplifies data collection and analysis with preconfigured protocols. Answer. DynaPro ™ Plate Reader III. minutes and begin reading microplates with seven mouse clicks. 000 to 2. NanoQuant plate. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. Plate Storage Solutions for Liquid Handling Automation Remote Lab Management. The color touch screen provides a visual user interface, making programming fast and intuitive. The entire Bulk Kit is sufficient for 100 plates (96- and 384-well), or 70 plates (1536-well). To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andthe iD5 reader’s filter system. Fluorescence Microplate Reader Comparison Chart. the Molecular Devices SPECTRAmax® 190 and 250. 5032338. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). U-bottom: 30-40 µl. The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. 5°C at 37°C. A = Log 10 (I 0 /I). QC tools and IQOQ services. Automated live cell imaging – cell counting and confluence. From the dropdown Menu, select Wellplates. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. e 3370 Costar 96 Well. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only. This SpectraMax Plus comes from a long history of reliable tools built by Molecular Devices. 96-well PCR plates are plastic, heat-resistant multi-well microplates designed for use inside PCR machines or real-time (qPCR) thermocyclers. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. Thermo Scientific Pierce Streptavidin Coated Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays. The eight-channel system delivers high precision and speed when reading 96-well microplates for single endpoint measurements and kinetic assay data gathered over time. ZERO BIAS - scores, article reviews, protocol conditions and moreThe VersaMax Absorbance Microplate Reader measures the optical density (OD) of samples at selected wavelengths in a number of reading modes. Growth medium was then replaced with fatty acid uptake solution. These include real-time PCR, measuring yields of RNA preparations for microarray experiments, reverse transcription PCR (RT-PCR), differential display PCR,. UV absorbance detection. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Click SoftMax Pro Software User Guide. 20 attomol ATP per well. Figure 3. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. Cross-talk <0. 313 reference gain check fail. Plate Background OD, which was used for subsequent tests, was measured as follows. M5. style. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. 100 ˜L (96-well) or 25 ˜L (384-well) of Loading Buffer was added to each assay well. where I 0 is the intensity of the incident light, and I is intensity of that light after it passed through the sample. Deep well blocks available in 24, 96 and 384-well formats An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. Kinetic assays were read every 20-28 seconds for one hour,The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. absorbance microplate reader GEA. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life. HeLa. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. To help you get the best results from your Molecular Devices® plate readers it is essential that you choose the right plate. 6. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. The plate reader optics are tunable for. The luminescence was measured in a SpectraMax. 100 fmol 1536-well (8 µL) 0. SoftMax Pro GxP Software version 5. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Molecular Spectroscopy Consumables & Accessories Thermal Analysis. Cell line development teams will realize savings on time and resources, as well as faster time-to-market by utilizing a single technology for measuring a critical quality attribute duringOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. 96-well microplate compatible Well read area range: D4. 3. The VICTOR Nivo. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. 340 PC384. 0. 01. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Microplates, cuvettes (via adapter) Detectors. Learn how you can utilize microplate readers to drive landmark discoveries. 2% in. 9000-0756. 2. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. OD measurements of bacterial and colloidal bead cultures were performed in a Spectrostar Omega microplate reader (BMG, Germany) with a Costar Flat Bottom 96-well plate with lid and 200 µl per. 96- and 384-well microplates. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. The VersaMax Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible. Both the P and M injectors were set to deliver 100 µL (96-well plate) or 25 µL (384-well plate), with a 2-second delay and 10-second integration time. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. This approach enables uniform light dose application to 9 wells (a 3-by-3 matrix in a 96-well plate) simultaneously, which can be leveraged to shorten the duration of PDT experiments (Fig. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. 50/plate. Polypropylene deep well plates, round bottom. Suggested plates are black walled. Unparalleled performance on a personalized platform. 190. Click “Next”, and the reader will start scanning the four corner wells; a progress indicator will appear: fter the reader finishes scanning the four corner wells, you will see a screen with an image of the upper left corner A well and a pink crosshair. the Bio-Tek PowerWave™ 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. This offers the potential for fast and convenient measurements of FAAH activity using a 96-well plate spectrophotometer. The four standard filters – 405, 450, 492, 620 nm – allow for a wide variety of ELISAs such as PNPP, ABTS, OPD and TMB. It is designed to work seamlessly with a variety of 96 well microplate readers, including popular brands such as Agilent™, Berthold™, Biotek™, BMG LABTECH™, Molecular Devices™, Perkin Elmer™, Promega™, Tecan™, and. Figure 1. Opaque-walled 96- or 384-well tissue culture plates are available as white or black with solid bottoms and white or black with clear bottoms. Morphology and fluorescence gates were set to select the best 96 clones secreting IgG antibodies. x. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. In addition, the iD5 reader measures TRF and FP and. Washers configured for both 96- and 384-well plates. • Easy and gentle immobilization of biotin. of 3 wells of a 96-well plate, from 1 of 3 independent experiments. D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. Plus 384. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. #C1500) Control standard endotoxin (ACC cat. B. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise. 96-well Molecular Devices P/N 42-000-0117 HE™ High Efficiency microplates, 384-well Molecular Devices P/N 0200-5201. SoftMax Pro Software minimizes setup time for detection and automates standard curve fitting and sample quantitation. 2. x. A = 2 - log 10 (%T). 4 (Molecular Devices cat. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. 0200-6263. Meanwhile, Cowan discusses the importance of considering plate well densities and the angle from which microplates are read inside the reader: “You should definitely check the range of plate well densities supported by your candidate readers. FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A) Flexibility to read a variety of plate formats. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. Published Dec 16, 2020 | Updated Jun 22, 2023. 000 ABS • Resolution: 0. Microplate Reader Ordering information Item Description Part number FLIPR Membrane. Absorbance Linearity: 2. The LysoTracker fluorescence at ex/em 574/594 was measured on a Spectramax i3x plate reader (Molecular Devices). The. We would like to show you a description here but the site won’t allow us. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range. Packed 25 plates per sleeve, 2 sleeves per case. 0 Optical system Filters Power requirementAfter reading the ELISA plate on the EMax® Plus Microplate Reader, the R2 value was 0. Make sure toPlate reader instructions Computer. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro densitometry of TLC plates and electrophoresis gels. This can increase throughput and efficiency, thus saving time and space in the lab. Fluorescence Microplate Reader Comparison Chart. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. D. 90349). Page 1 of 8 . Please click on the links below to view the Microplate Reader Comparison Tables. . Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. 130009WMicroplate Readers. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. 1. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. Fluorescence Microplate Reader Comparison Chart. A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. Each plate reader or washer is calibrated, inspected, and lubricated—any potential issues . 315 can't find zero order. PURPOSE. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. 1. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). #E010) Pyroplate 96-well microplate (ACC cat. x. 000 to 2. When a. It features fully-automated kinetic reads of the 96-well plate via the WinKQCL TM Software. Silica excitation fiber, glass emission fiber. To validate the assays’ ability to detect a wide range of viable cell numbers, a series of cell densities was set up. The SpectraMax Paradigm Platform was able to quantify the protein down to the low picomolar level using an AlphaLISA method (Figure 1). Quartz Plate found in: Thick Quartz Ground and Polished Plates, Solid Quartz 96 Well Clear Plate with Clear Base, Quartz Parallel Plate Probes for DMA 7. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Microplate format. It features a modular design that allows users to add or change detection cartridges according to their needs. 1. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. 300 µL of Detergent Compatible Bradford reagent was added to each well. Unparalleled performance on a personalized platform. SUPPORTED PLATES Microplates having 6, 12, 24, 48, 96, and. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. 304 signal level saturation. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Absorbance equation. Uncontrolled Copy for Training and Reference Purposes Only . SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr: $32/hr. 000 OD ± 1. 001 ABS (displayed), 0. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. $488. 0025 OD from 0. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of the human AT1R receptor. These microplates have a corner notch for use in standard plate readers. 046 to 0. What are the dimensions of the plate? Code. Ensure ongoing compliance of your Molecular Devices microplate readers and be audit ready with comprehensive . We would like to show you a description here but the site won’t allow us. Temperature variation ± 0. 9. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. *For Corning UV plates, mean values at 260 nm ranged from 0. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. Products : Ionworks HT, Ionworks Quattro. With various on-board features and capabilities, the ThermoMax. Microplate optimization and read height optimization were performed for best results. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. For FlexStation I/II/3 plate readers, what are the dead-volumes for the compound plate? The FlexStation plate readers assume a 1 mm pipette height from the bottom of the well when aspirating from a compound source plate. Instrument settings can beIntegrating ValitaTiter and Molecular Devices fluorescence polarization (FP) plate readers produces a seamless IgG titer assay setup and optimization. #3693; n = 4 for each standard). 306 plate air cal. x. Please refer to the Obsolete Microplate Reader Models for more information. Self-righting base, up to 40 degrees past center without dispenser. Microplate optimization and read height optimization were performed for best results. 005 OD Normal and Rapid modes, 96- and 384-well plates;. Published Dec 16, 2020 | Updated Dec 16, 2020. The procedures in this protocol support the 96-well reaction plate. 96-well plates - Filter plates with 0. For biochemical assays, the washer removes unbound material and unreacted reagents. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. Our washers provide speed and configurability options—from single-tube dispensing to washing full 96- and 384-well plates. minutes and begin reading microplates with seven mouse clicks. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. The CellTiter-Glo assay was used to measure ATP. It also integrates with the powerful SoftMax Pro Software for. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Supplier: Molecular Devices. It has a 340-750nm Spectral Range. White light and fluorescence images of the 6-well plates were captured with the ClonePix 2 System. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into cardiomyocytes and neurons, up to 100 times per. FlexIII. 96- & 384-well automated washer, multiwash™+ automated washer, 96- and 384-well plates, washer maintenance kit.